Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules
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چکیده
منابع مشابه
Safer staining method for acid fast bacilli.
To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...
متن کاملThe staining of bacterial capsules and slime with ferric compounds.
In 1946 Hale described a method for demonstrating acid mucopolysaccharides in sections based on the affinity of these compounds for colloidal iron oxide in acid solution. The combined iron was demonstrated by conversion to prussian blue. It has been found that bacterial capsules and slime are very well stained by Hale's method. This is possibly due to their content of acid mucopolysaccharides, ...
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Immersion of tissue in aqueous uranyl acetate be-for giving some degree of contrast to components fore embedding is commonly practiced to increase such as membranes when they extend through the the contrast on sections which are subsequently thickness of the section parallel to the electron stained (2, 3, 5). The treatment is only satisfactory beam. A simple modification of current practice 540
متن کاملThe demonstration of bacterial capsules by Fontana's staining procedure.
It has been discovered that, in addition to bacterial cells being stained a very dark brown or black color by Fontana's procedure, bacterial capsules are differentially stained a light brown color. Fontana (Dermatol. Wochschr., 55, 1003, 1912 and Pathologica, Genova, 4, 582, 1912) described a method for staining Treponema pallidum and other spirochetes by the AgNO3NH40H method. A similar proced...
متن کاملA Simple Method for Staining the Capsules of Bacteria.
The possession of mucinous envelopes by certain bacteria, apart from their purely morphological interest, has a great value in the identification of such organisms and many methods have been devised for their demonstration. The number of such methods however argues against any one being completely satisfactory. The introduction of a new technique into this already overcrowded field can be justi...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2015
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927615004407